OBSERVATION OF THE MICROFILAMENTS IN THE CULTURED HUMAN FIBROBLASTS WITH COOMASSIE BLUE R 250
- VernacularTitle:用考马斯蓝R250(Coomassie Blue R250)显示人成纤维细胞内微丝的观察
- Author:
Menglian ZHAO
;
Minying NIU
;
Baorong FAN
;
Jie GAO
;
Kunren WANG
;
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The experiments was conducted on the fibroblasts of human prepuce in vitro todemonstrate the distribution of cytoplasmic microfilaments with Coomassie Blue R250.It has been found that the distribution of the cytoplasmic microfilaments canbe disturbed by the specific inhibitor,Cytochalasin B.We also observed the inhi-bition by Cytochalasin B was reversible.The cytoplasmic microfilaments regainedtheir normal distribution after the Cytochalasin B was removed.This result further confirmed that the staining method of Coomassie BrilliantBlue R 250 is reliable to demonstrate microfilaments in cultured fibrablasts.
