The Effects of GAPS on Inhibiting Gastric Cancer Cell Line MGC -803 Proliferation and PDGFR - B Expression
- VernacularTitle:树舌多糖抑制胃癌MGC-803细胞增生及对PDGFR-?表达的影响
- Author:
Ronghui LI
;
Yu WANG
;
Lihong ZHENG
;
Chunjing ZHANG
;
Huiyu XU
;
Rui WANG
;
Yingbo ZHANG
;
- Publication Type:Journal Article
- Keywords:
GAPS;
MGC -803 cell;
Proliferation;
PDGFR-?
- From:
Journal of Medical Research
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of ganoderma applanatum polysaccharides(GAPS) on cell morphology, proliferation and PDGFR -?expression in cell lines MGC - 803 , and to explore its potential mechanisms of anti - tumor of GAPS. Methods Cell morphology was observed by inverting microscope. MTT assay was used to investigate the inhibitory effect of GAPS on MGC -803.Expressions of PDGFR -?was analyzed by flow cytometry (FCW). The fluorescence intensity of expressions of PDGFR -?was observed by fluorescence microscope. Results Cells of GAPS group were irregular shaped and grew poorly. GAPS inhibited the proliferation of MGC -803 cells in dose - dependent and time - dependent manners . After MGC - 803 cells were treated with GAPS for 72h, GAPS could down - regulate expression of PDGFR -?observed by fluorescence microscope which was consistented with the results of FCW with statistic significance difference as compared with control group (P
