Expression of Pax6 gene in retinoblastoma
- VernacularTitle:眼发育调控基因Pax6在视网膜母细胞瘤中表达的初步研究
- Author:
Hui-Juan WANG
;
Bin LI
;
Hao ZHANG
;
Yu-Zhi AN
;
Zhi-Qiang PAN
;
- Publication Type:Journal Article
- Keywords:
retinoblastoma;
Pax6;
reverse transcriptase polymerase chain reaction;
gene expression
- From:
Ophthalmology in China
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the gene expression of Pax6 in retinoblastoma (Rb).Design Experimental study.Participants Six cases of fresh Rb tumors and two established Rb cell lines.Six cases of retina tissues as normal control group.Methods Pax6 gene ex- pression was assessed in two established Rb cell lines,six primary tumors and six cases of retina tissues using the reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot.Main Outcome Measures Expression of Pax6 gene in fresh retinoblastoma tis- sue and cell lines.Results The mRNA of Pax6 gene was positive in all two established Rb cell lines and the six primary Rb tumors using RT-PCR.The protein of Pax6 gene was also positive in Western blot.Pax6 mRNA and protein expression in the Rb tumors were increased compared with normal control group (P
