Neuronal apoptosis and expression of caspase-12 mRNA and protein following focal cerebral ischemia-reperfusion in rats
- VernacularTitle:大鼠脑缺血/再灌注后神经元凋亡及caspase-12 mRNA和蛋白表达的改变
- Author:
Hong ZHANG
;
Li-Chun SONG
;
Chun-Hong JIA
;
Yan-Yan LIU
;
Yong-Li LV
;
- Publication Type:Journal Article
- Keywords:
cerebral ischemia;
apoptosis;
caspase-12;
endoplasmic reticulum
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the expression of caspase-12 mRNA and protein following focal cerebral ischemia-reperfusion in rats,and explore the effect of endoplasmic reticulum pathway on neuronal apoptosis.Methods 60 male Wistar rats were randomly divided into sham-operated group and ischemic group.The middle cerebral artery occlusion(MCAO)models were established by using the intraluminal suture occlusion method,neuronal apoptosis was detected by TUNEL staining,the expression of caspase-12 protein was detected by immunohistochemical staining,the expression of caspase-12 mRNA was detected by RT-PCR method.Results In ischemic group,the number of apoptotic cells and the expression of caspase-12 mRNA and protein were gradually increased following prolonged cerebral reperfusion,reached the peak at 24 h.The number of apoptotic cells and the expression of caspase-12 mRNA and protein in ischemic group were significantly less than those of sham-operated group at all times(P
