The influence of erythropoietin to the ultrastructure of retinal neurons and content of retinal glutamate in the early stage diabetic rats
- VernacularTitle:促红细胞生成素对早期糖尿病大鼠视网膜谷氨酸含量和超微结构的影响
- Author:
Bi-Jun ZHU
;
Wei-Jun WANG
;
Xun XU
;
- Publication Type:Journal Article
- Keywords:
diabetic retinopathy;
erythropoietin;
glutamate;
animal model
- From:
Ophthalmology in China
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficacy of recombinant human erythropoietin(rhEPO)in preventing and reversing dys- function of retinal neurons in streptozotocin(STZ)-induced diabetes in rats.Design Experimental study.Participants The early stage of diabetic rats.Method Two weeks after STZ(60 mg/kg,i.p.),diabetic rats were administered rhEPO(5000IU/kg)injection three times weekly for 2 weeks.Retinal samples of STZ-induced diabetic rats with or without rhEPO and controls were prepared for ultrathin sec- tions and subsequently photography by transmission electron microscope.Also the content of glutamate in retina of the rats was detected with high-performance liquid chromatography.Main Outcome Measures The ultrastructure of rat retinas and the content of retinal glutamate.Results Mitochondrial metamorphosis in ganglion cells occurred in STZ-induced diabetic rats without rhEPO.Obvious mito- chondrial metamorphosis couldn′t be found in STZ-induced diabetic rats with rhEPO treatment.Retinal glutamate at the end of the 4th week of STZ-induced diabetic rats had increased obviously comparing with the normal rats(P
