Effects of estradioi on radiation-induced apoptosis of bone marrow hematopoietic cells in mice
- VernacularTitle:苯甲酸雌二醇对辐射诱导小鼠骨髓造血细胞凋亡的影响
- Author:
Bai-Long LI
;
Jian-Ming CAI
;
Jian-Guo CUI
;
Ying-Song XIANG
;
Fu GAO
;
Ru-Jun YANG
;
Yue-Cheng HUANG
;
- Publication Type:Journal Article
- Keywords:
extradiol;
hematopoietic cell;
apoptosis;
radiation injuries;
Fas;
Bcl-2
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of estradiol on ~(60)Co?-ray induced apoptosis of bone marrow hematopoietic cells of mice,and to discuss the related anti-irradiation mechanism.Methods:KM mice were randomly divided into 3 groups(15 mice/each group):control group(without radiation),pure radiation group and estradiol+radiation group(ER group).The pure radiation group was irradiated by 4.0 Gy?-ray at a dose rate of 1.15Gy/min;the ER group was administered with 0.1 mg estradiol(IM)at 10 days before 4.0 Gy?-ray radiation;and the control group received no special treatment.The apoptotic DNA segments of bone marrow hematopoietic cells were analyzed by DNA agarose gel electrophoresis;flow cytometry was used to examine the apoptosis rate of cells and expression of Fas and Bcl-2 at 4 h,8 h,and 12 h after irradiation.Results:Eight hours after radiation,the apoptotic DNA segments were obviously increased and apoptotic DNA ladder appeared,which was not seen in the other 2 groups.The apoptosis rate of bone marrow hematopoietic cells in ER group was significantly lower than that in the pure radiation group at 4,8,and 12 h after irradiation(P
