Microsatellite instability and loss of heterozygosity on chromosome 3p,9p and 14q in renal cell carcinoma
- VernacularTitle:肾细胞癌中染色体3p、9p、14q微卫星不稳定及杂合性缺失分析
- Author:
Hai-Tang CHEN
;
Wen-Jun CHANG
;
Hong-Yu YU
;
Jin-Feng ZHAO
;
Guang-Wen CAO
;
- Publication Type:Journal Article
- Keywords:
kidney neoplasms;
renal cell carcinomas microsatellite instability;
loss of heterozygosity
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate frequencies of microsatellite instability(MSI)and loss of heterozygosity(LOH)in renal ceil carcinoma(RCC),and to discuss the relationship of clinicopathological characteristics of RCC with MSI and LOH. Methods:Twelve microsatellite markers located at chromosomes 3p,9p and 14q were selected to investigate microsatellite alterations(MSI and LOH)in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction- polyacrylamide gel elect rophoresis-ethylene dibromide(PCR-PAGE-EB)staining and sequencing.Results:The frequency of MSI could reached 61.3% and that of LOH could reach 54.8%.The highest frequency of MSI was at locus of D9S168(32.3%);the highest frequency of LOH was at locus of D3S1289(21.4%).No correlation was found between MSI or LOH and the patients' age,sex,pathology type and metastastis,except that MSI was correlated with TNM stage of RCC(P
