Dihydromyricetin induce apoptosis in AGZY-83-a tumor cells
- VernacularTitle:二氢杨梅素诱导人肺腺癌细胞系AGZY-83-a凋亡的实验研究
- Author:
Mei-Mei YIN
;
Zhen-Wei PAN
;
Ben-Zhi CAI
;
Hong-Li SHAN
;
Da-Yan HUANG
;
Yan-Jie LV
;
Bao-Feng YANG
;
- Publication Type:Journal Article
- Keywords:
dihydromyricetin;
apoptosis;
AGZY-83-a cells;
Ca~(2+)
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of dihydromyricetin on the apoptosis in AGZY-83-a tumor cells and its relevant mechanisms.Methods The survival rate of AGZY-83-a cells was assayed by MTT dye reduction.The cellular apoptosis was analyzed by TUNEL method and caspase-3 activity.The intracellular free calcium i was detected to explore the apoptotic mechanism of dihydromyricetin.Results In the MTT assay,dihydromyricetin 50?mol?L-1 significantly inhibited survival ratios of AGZY-83-a cells at dose-and time-dependent manner.It was demonstrated in TUNEL assay that dihydromyricetin could induce the apoptosis of AGZY-83-a cells in a concentration-dependent manner.The examination of Caspase-3 activity indicated that the dihydromyricetin could induce the apoptosis of AGZY-83-a cells,which was dose-dependent activation of Caspase-3 in AGZY-83-a cells.The detection of the intracellular i showed that the average FI of the i could be markedly increased to 20-fold as the basic condition.Conclusions Dihydromyricetin can induce the apoptosis in AGZY-83-a cells,which is associated with the overload of the intracellular i.
