Expression of HIF-1? and its relationship with angiogenesis in osteosarcoma
- VernacularTitle:低氧诱导因子1?在骨肉瘤中的表达及其与血管生成的相关性
- Author:
Qing-Cheng YANG
;
Bing-Fang ZENG
;
Zhi-Chang ZHANG
;
Zhong-Min SHI
;
Yang DONG
;
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
hypoxia-inducible factor-1?;
angiogenesis
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of HIF-1?and its relationship with angiogenesis in osteosarcoma.Methods: Osteosarcoma MG-63 cells were cultured in vitro under hypoxia and mimic hypoxia conditions.Thirty paraffin-embedded osteosarcoma tissues and 20 fresh frozen osteosarcoma specimens were collected.The mRNA and protein expression of HIF-1?and VEGF were detected by RT-PCR,Western blotting,ELISA,and immunohistochemistry methods.The mean vessel density(MVD)were also calculated.Results:The mRNA level of HIF-1?had no change under hypoxia and minic hypoxia conditions,whereas the protein expression was increased dramaticaly.The mRNA and protein expression of VEGF was significantly increased under hypoxia and minic hypoxia conditions.The positive rate of HIF-1?mRNA(90%)and VEGF(100%)in 20 fresh frozen tissues were higher than those of the para-tumor tissues(P
