Construction and sequence analysis of the recombinant plasmid affecting gene ATX translation by RNA interference
- VernacularTitle:ATX短发夹shRNA表达载体的构建和测序
- Author:
Ying-Hai XIE
;
Bin LIU
;
Ai-Ming WANG
;
- Publication Type:Journal Article
- Keywords:
ATX gene;
RNA interference;
Short hairpin RNA;
Recombinant plasmid
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the recombinant plasmid carrying shRNA to ATX and analyze the nucle- ic acid sequence for further searching new gene therapy method of tumor.Methods Two DNA sequences containing short hairpin structure were designed and synthesized.The complement form was obtained by annealing and inserted into vector Psilcncer2.1-U6 neo,and the recombinant plasmid was transformed into DH5a strain.Finally the plasmid identified by restriction enzyme was used for sequence analysis.Results The recombinant Psileneer2.1-U6 neo car- rying shRNA to ATX had been constructed and the aim sequence had been obtained.Conclusion The construction of the recombinant plasmid carrying shRNA to ATX lays the basis for the study of its inhibitive effect on tumor.
