Regulatory effects of 3 kinds of antifibrotic cytokines on activity of transforming growth factor-?_1 gene promoter
- VernacularTitle:三种抗纤维化细胞因子对HepG2细胞转化生长因子-?_1基因启动子转录活性的影响
- Author:
Li-Na HOU
;
Chun-Fang GAO
;
Yun-Peng ZHAO
;
Xiao-Juan SUN
;
Jian-Dong CHEN
;
Xiao-Jiong JIANG
;
- Publication Type:Journal Article
- Keywords:
polymorphism;
single nucleotide;
interleukin-10;
hepatocyte growth factor;
interferon-gamma;
transforming growth factor beta;
transcription;
genetic;
liver cirrhosis
- From:
Academic Journal of Second Military Medical University
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
T polymorphism,was selected as putative promoter.The recombinant constructions containing-1328-+812 of TGF-?_1 gene and CAT reporter gene(phTGF2.14T,phTGF2.14C)were constructed and transfected into HepG2 cells with liposomal trans- fection method,then the transfected HepG2 cells were treated with IL-10(4 ng/ml),HGF(10 ng/ml)or IFN-?(20 ng/ml). Reporter gene activity was analyzed by ELISA.Results:Reporter gene activity in cells transfected with phTGF2.14C was sig- nificantly higher than those transfected with phTGF2.14T(P
