Toll like receptor 9 expression and functions of plasmacytoid dendritic cells in the peripheral blood of patients with chronic hepatitis B virus infection
- VernacularTitle:慢性乙型肝炎病毒感染者外周血浆样树突状细胞Toll样受体9的表达和分泌功能
- Author:
Ni-Na JIA
;
Qing XIE
;
Bao-Yan AN
;
Lan-Yi LIN
;
Huai-Cheng SHEN
;
Hui WANG
;
Xia-Qiu ZHOU
;
Hong YU
;
Qing GUO
;
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Dendritic cells;
Receptors,cell surface;
Oligonucleotides;
Interferon-alpha
- From:
Chinese Journal of Infectious Diseases
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Toll-like receptor(TLR) 9 of circulating plasmacytoid dendritic cell (pDC) and analyze the frequency and interferon (IFN)-?production of circulating pDC during hepatitis B virus(HBV) infection.Methods Peripheral blood was collected from 69 HBV-infected patients,including 14 cases of asymptomatie HBV infection,30 cases of chro- nic hepatitis B(CHB),25 cases of HBV-related liver cirrhosis,and 21 healthy blood donors as con- trols.Flow cytometry was used to analyze the frequency of circulating pDC and the mean fluorescence intensity(MFI) of TLR9.Fresh peripheral blood mononuclear cells(PBMC) were stimulated by CpG ODN 2216 for 24 h in vitro.IFN-?in the supernatant was measured using enzyme-linked immunosorbent assay(ELISA) to analyze the frequency and IFN-?production of pDC during HBV in- fection.Data were analyzed with SPSS 13.0 for windows.Results Compared with healthy controls (62.6?10.7),the MFI of TLR9 of patients with asymptomatic HBV infection,those of CHB and HBV-related cirrhosis were significantly reduced (P
