The influences of hepatitis B virus precore and basic core promoter region mutations on the immune responses of specific cytotoxic T lymphocytes
- VernacularTitle:乙型肝炎病毒前C/BCP区基因变异对特异性细胞毒性T淋巴细胞免疫应答的影响
- Author:
Xiao-Juan YANG
;
Guo-Rong WU
;
Hao PEI
;
Jin-Juan QIAN
;
Rui-Yun JI
;
- Publication Type:Journal Article
- Keywords:
Genes,viral;
Variation(genetics);
Hepatitis B,chronic;
T-lymphocytes,cytotoxic
- From:
Chinese Journal of Infectious Diseases
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influences of mutation at precore and basic core promoter(BCP) region in hepatitis B virus(HBV) on the immune response of specific cytotoxic T lymphocytes(CTL) in patients with chronic hepatitis B(CHB).Methods The number of specific CTL in peripheral blood mononuclear(PBMC) of CHB patients were tested by cytokine flow cytome- try(CFC) and HBV core18-27 peptide.HBV precore and BCP fragments were directly sequenced. Results Twenty-one(38.9%) samples were HBV precore G1896A mutation.Twenty-six(48.1%) samples were BCP region 1762/1764 combined mutation.Thirteen(24.1%) stains were three sites mutated simultaneously.Stimulated with HBV core 18-27 in vitro,the specific CTL level was signifi- cantly higher in the patients with G1896A mutation and BCP region mutation [(0.41?0.09)%, (0.36?0.08)%,(0.48?0.08)%,respectively]than those without mutation[(0.11?0.06)%, P
