The experimental study of histology and apoptosis after the bone cement leakage into the intervertebral disc
- VernacularTitle:骨水泥椎间盘渗漏后组织学和细胞凋亡的实验研究
- Author:
Hui ZHAO
;
Cai-Fang NI
;
Long CHEN
;
Tian-Si TANG
;
Hui-Lin YANG
;
Jian-Fei HUANG
;
- Publication Type:Journal Article
- Keywords:
Lumbar vertebrae;
Immunohistochemistry;
Polymethacrylic acids;
Calcium phosphates
- From:
Chinese Journal of Radiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the histology change,apoptosis state and Bcl-2,Bax expression after the bone cement leakage into the intervertebral disc in vertebroplasty.Methods Eight majority canis familiaris were studied.Three lumbar intervertebral discs(L2 to L5)in each dog were randomly classified into three groups(control group,PMMA group,and CPC group),the canine discs were stabbed by 18-gauge needle,and 0.1 ml cement was injected into them.Control discs were only stabbed and injected with nothing.Histology of all discs was studied 24 weeks after the operation.Terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL)and immunohistochemisty were used to detect apoptosis and Bcl-2,Bax expression in the discs.The data were statistically analyzed by SPSS 12.0.Results Intervertebral disc degeneration was not found in control groups.In bone cement groups,however,ruptured or serpentine patterned fibers,decreased cellularity of the nucleus pulposus and condensed matrix of the nucleus pulposus were found in histologic results.The Bax protein decreased in the order of control group, CPC group,and PMMA group.However,the Bcl-2 protein increased in the order of control group,CPC group,and PMMA group.The histology grade was significantly different among the three groups under ANOVA analysis(P
