The expressions and their relationship of PTTG,P27 and CDK4 in glioma
- VernacularTitle:垂体瘤转化基因、P27及周期素依赖性激酶4在胶质瘤中表达及其相关性研究
- Author:
Yu-Huan YUAN
;
Ru-Fei DAI
;
Jun CAI
;
Xiao-Ming LI
;
Chao YAN
;
- Publication Type:Journal Article
- Keywords:
Glioma;
Oncogenes;
CDC_2 protein kinase
- From:
Chinese Journal of Primary Medicine and Pharmacy
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of expressions of pituitary tumor transforming gene(PTTG) and p27 in glioma,and explore the relationship between the expressions of them and cell cycle regulation.Methods Specimens of 40 patient with gliomas were divided into gradeⅠ:8 cases,gradeⅡ:10 cases,gradeⅢ:13 cases, gradeⅣ:9 cases,PTTGmRNA and p27mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR),and their expressions of PTTG,P27,CDK4 protein were detected by immunostaining assay using strep- tavidin-peroxidase(SP) method.Results The expressions of PTTGmRNA in gradeⅠ～Ⅳwere (0.907?0.065),(1.109?0.083),(1.312?0.089),(1.499?0.215) respectively,there was significant difference among the groups(P
