Assessment of the reliability of haman corneal endothelial morphologic parameters estimated using center by center method analysis
- VernacularTitle:应用简化细胞分析软件进行角膜内皮细胞图像定量分析的可靠性研究
- Author:
Xuan LIU
;
Ping ZHU
;
- Publication Type:Journal Article
- Keywords:
specular microscopy;
corneal endothelium;
quantitative morphometric analysis
- From:
Ophthalmology in China
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect on reliability with different sample amount when human corneal endothelial morpho- logic parameters estimated using center by center method analysis.Design Clinical study.Participants Thirty central corneal endothe- lium photo-micrographs for the same eye. Methods Noncontact specular photomicrographs were obtained.Each photo-micrograph was analyzed with center by center method and was analyzed for 9 times by pointing different amount cells,from 10 to 90.Compare the di- versity of corneal endothelial morphometric parameters between different groups. Main Outcome Measures Corneal emtothelium den- sity,coefficient of variation.Results The coefficient of variation decreased and then kept to a same level when sample cell amount were added from 10 to 90.With the sample cell amounts varied from 10,20,30,40,50 to 90,the coefficient of variation was changed from 9.5,6.3,4.6,4.4.,4.3 to 3.8.Corneal endothelium density had significant difference between the first two groups of 10 and 20 cells with the other groups(P
