Experimental study on systemic hematogenic immunoreactions activated by bacteria in simulation of natural system
- VernacularTitle:细菌激活系统血液免疫反应仿真自然体系实验研究
- Author:
Feng GUO
;
Le-Zhi ZHANG
;
Pin-E GUO
;
Yu-Lian XU
;
Mei-Xian HUA
;
Bao-Hua QIAN
;
- Publication Type:Journal Article
- Keywords:
Yeasts;
Erythrocytes;
Hematogenic immune reaction;
Completement 4;
Interleukin-8
- From:
Chinese Journal of Infectious Diseases
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study systemic hematogenic immunoreactions induced by bacterial infections using simulation of natural system.Methods Whole blood 0.2 mL or white blood cells 0.2 mL and plasma(or normal saline)0.3 mL were stimulated by 0.2 mL of yeast and inactivated Bacillus Calmette-Guerin(BCG,5?10~8/mL),respectively,which were incubated at 37℃for 1 h. Interleukin(IL)-8,C3,C4 and chemokine receptor Fy6 were detected by flow cytometry(FCM)and en- zyme-linked immunosorbentassay(ELISA).Results Bacteria could activate red blood cell to modulate IL-8 release from white blood cells in plasma.In nature experimental group,activation rate(37.04?34.84)of IL-8 was significantly higher than that(1.09?0.77)in isolation experimental group.In nature experimen- tal group,value increment(0.01?0.01)of complement C4 was significantly higher than that(-0.0027?0.008)of isolation experimental group(P
