Expression of human gene 5 transactivated by pre-S1 protein of hepatitis B virus in yeast cell
- VernacularTitle:乙型肝炎病毒前S1蛋白反式激活基因5在酵母细胞中的表达
- Author:
Jian-Kang ZHANG
;
Jun CHENG
;
Jiang GUO
;
Yong-Zhi LUN
;
Dan-Qiong WANG
;
Long-Feng ZHAO
;
Yuan HONG
;
Yu MAO
;
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Pre-S1 protein;
Transactivated protein;
Yeasts;
Gene expression
- From:
Chinese Journal of Infectious Diseases
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the exact function of human gene 5 transactivated by pre-S1 protein of hepatitis B virus(PS1TP5)by investigating the gene expression of PS1TP5 in yeast cells. Methods Reverse transcription-polymerase chain reaction(RT-PCR)was performed to amplify the gene of PS1TP5 using the mRNA of HepG2 cells as template and the gene was cloned into pGEM-T vector.The gene of PS1TP5 was cut from pGEM-T-PS1TP5 vector and cloned into yeast expressive plasmid pGBKT7,then pGBKT7-PS1TP5 was transformed into yeast cell AH109.The yeast protein was isolated and analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Western hybridization.Results PS1TP5 gene was successfully amplified and identified by DNA sequencing.The digested fragment was cloned into pGBKT7 vector and transformed into yeast cell AH109.The results of SDS-PAGE and Western assay showed that the relative molecular weight of the expressed product was about 36 950,and PS1TP5 protein existed in yeast cells.Conclusion The findings suggest that PS1TP5 can be successfully expressed in yeast cell.
