The effect of adenover-mediated VEGF gene on surival of a random skin flap in the rat
- VernacularTitle:复制缺陷型腺病毒载体介导的VEGF基因治疗大鼠缺血皮瓣的研究
- Author:
Yuan YAO
;
Chun-Lan WANG
;
Bang-He WANG
;
- Publication Type:Journal Article
- Keywords:
Gene;
Vaseular endothelial;
Growth Factor;
Adenovirus;
Skin flap
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of adenovirus-medicated gene therpy with vascular endothelial growth factor(VEGF)delivered into the subdermal space to treat compromised skin flap in the rat.Methods Thir- ty sprague-dawley rats weighing 350～400g were used in this study.The rats was randomly divided into three groups of ten rats each.A dorsal flap(8cm?2cm)in full thickness with the pedicale located at the leve of the iliaccrest was designed,each,animal received recipient bed subfascial injections of adenovirus encoding VEGF(Ad-VEGF)or green fluorescent protein(Ad-GFP)as treatment control.Another set of animals(n = 10)received NG was designated as control.The flap were elevated as originally designated and sutured back to its bed.The survival rate of the flap was evaluated on day seven after operation.Results The survival rates of the flap in Ad-VEGF group increased signifi- cantly as compared with those of the Ad-GFP group(P
