Glypican-3 expression in hepatocellular carcinoma by RT-PCR and SSCP.
- VernacularTitle:Glypican-3基因在肝细胞癌组织中的表达
- Author:
Gui-Lin XIE
;
Min ZHOU
;
Mu-Sheng LIN
;
Shi-Ting BAO
;
Hui-Lai MIAO
;
- Publication Type:Journal Article
- Keywords:
Glypican-3 gene;
Carcinoma,hepatocellular;
Mutation
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate Glypican-3 gene expression and mutation in hepatocellular carcinoma (HCC).Methods Glypican-3 gene expression and mutation in tumor,para-c.ancer and normal tissue of 48 HCCs were detected by RT-PCR and single-strand conformation polymorphism(SSCP),respectively.Results There was no Glypican-3 mRNA expression in para-cancer and normal tissue.Expression rate of Glypican-3 mRNA was 77.1% in tumor tissue,which was correlated with clinical staging and cell differentiation(P
