The Antiproteinuric Effects of Green Tea Polyphenol on Cyclosporine A-Induced Acute Renal Injury in Mice.
- Author:
Byung Chul SHIN
1
;
Hyun Lee KIM
;
Jong Hoon CHUNG
Author Information
1. Department of Internal Medicine, College of Medicine, Seonam University, Gwangju, Korea.
- Publication Type:Original Article
- Keywords:
Green tea polyphenol;
Cyclosporine;
Proteinuria;
Antioxidant
- MeSH:
Acute Kidney Injury*;
Animals;
Cyclosporine*;
Guanosine Triphosphate;
Injections, Subcutaneous;
Kidney;
Lipid Peroxidation;
Malondialdehyde;
Mice*;
Nitric Oxide;
Proteinuria;
Tea*
- From:Korean Journal of Nephrology
2006;25(6):913-921
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND:It has been reported that there is association between cyclosporine (CsA) nephrotoxicity and proteinuria. The aim of this study was to investigate the anti-proteinuric effects of green tea polyphenol (GTP) on CsA-induced acute renal injury in mice. METHODS:The mice (n=20) were divided into 4 groups (n=5/group); group 1 (control group) mice were intraperitoneally (IP) injected 0.9% saline, group 2 (CsA group) mice were IP injected CsA 50 mg/kg, group 3 (iNOS inhibitor group) mice were given in addition N(G)-nitro-L-arginine-methyl ester (L-NAME) 12 mmol/L by subcutaneous injection. Group 4 (GTP group) mice were given CsA by IP injection and GTP 100 mg/kg by subcutaneous injection. RESULTS:Urine protein significantly increased in group 2 (28.6+/-11.1 g/kg/day) compared to group 1 (9.1+/-5.5 g/kg/day, p<0.01) and significantly decreased in group 4 (11.2+/-8.8 g/kg/day, p<0.01) compared to group 2. Renal tissue malondialdehyde level of group 2 significantly increased compared to group 1 and significantly decreased in GTP group (p<0.01). CONCLUSION:This study proves that proteinuria of the CsA induced nephrotoxicity is associated with lipid peroxidation and nitric oxide production. GTP treatment has meaningful antiproteinuric effects through antioxidative effect in the kidney from CsA-induced acute renal injury.
