Study on human leukocyte antigen-[ restriction sites in human immunodeficiency virus type 1 specific CD8~+T cell responses
- VernacularTitle:人类免疫缺陷病毒1型特异性CD8~+T细胞应答中人类白细胞抗原Ⅰ的限制位点
- Author:
Yang-Bo TANG
;
Xiao-Ping TANG
;
Xia JIN
;
- Publication Type:Journal Article
- Keywords:
HIV 1;
CD8-positive T-lymphocytes;
HLA antigens;
Restriction site;
Epitopes;
Enzyme-linked immunospot assay
- From:
Chinese Journal of Infectious Diseases
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate human immunodeficiency virus type 1 (HIV 1)specific CD8'T cell responses and related human leukocyte antigenⅠ(HLA-I)restriction sites.Methods Four Epstein-Barr virus (EBV)strains-transformed B-lymphoblastoid cell lines (BLCL)from periph eral blood monocytes (PBMC)of 4 healthy individuals were established.Using synthesized peptides in HIV-1 Gag,Vif and Nef regions as epitopes and using BLCL as antigen presenting cells (APC), the frequency of interferon-7(IFN-?)secreting cells in CD8 T cells from a HIV-infected long term nonprogressor by enzyme-linked immunospot (ELISPOT)assay in vitro were assessed.Moreover, matching analysis of HI.A-Ⅰrestriction sites were performed.Results HIV-1 epitopes were presen ted restrictively by HLA-Ⅰmolecules in specific CD8 T cell responses,and this antigen presenting had high specificity.HLA-Ⅰrestriction sitesA03,A26,C04andC08 contributed to presentepitopes in HIV-1 C-ag region,A03 and C08 to HIV-1 Nef as well.Conclusion HLA-Ⅰrestriction sites may include A03,A26,C04 and C08 in HIV-1 Gag region,and A03,C08 in Nef region.
