Establishment of serological detection methods of 3 types of human herpesvirus 8 specific-antigens and complex antigens
- VernacularTitle:三种人类8型疱疹病毒特异性抗原表达及复合抗原血清学检测方法的建立
- Author:
Xing WANG
;
Fang-Ping HE
;
Xiao-Mei LU
;
Shu-Jun ZHAO
;
Ren-Yong LIN
;
Bin HE
;
Hao WEN
;
- Publication Type:Journal Article
- Keywords:
Sarcoma,Kaposi;
Herpesvirus 8.human;
Gene expression;
Antigens;
Enzyme linked irnmunosorbent assay;
Recombinant fusion protein
- From:
Chinese Journal of Infectious Diseases
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish serological detection methods of human herpesvirus 8 (HHV-8).Methnds Three potent antigenic fusion proteins.K8.1,ORF65 and ORF73 C of HHV- 8 were synthesized using E.coli system.The sera were detected using lhese antigenic proteins.The positive sera were from 12 patients with Kaposi's sarcoma and 32 patients with acquired immunodeficiency syndrome-related Kaposi's sarcoma.The negative sera were from 20 patients with cutaneous tumors and children under 15 years old.Western blot and enzyme-linked immunosorbent assay (EI.ISA)were employed to determine the immunogenicity of each recombinant protein and the sensitivity and specificity of ELISA using the complex antigens.Results Three types highly purified HHV 8 specific recombinant pro teins with potent antigenicity were successfully synthesized.The sensitivity of ELISA using the above complex antigens was significantly higher than traditional immuno-flurescent assay (IFA)detecting the positive and negative sera,whieh were 81.8%,34.4%,respectively.And the specificity of ELISA was 97.9%.Conclusion K8.1,ORF65 and ORE73 C are good candidate antigens for establishing HHV-8 serological detection methods,which have better sensitivity and specificity.
