The preliminary study on molecular biologic staging of non-small cell lung cancer lymph nodes
- VernacularTitle:肺癌淋巴结分子生物学分期的初步研究
- Author:
Yuan QIU
;
Jian-Xing HE
;
Han-Zhang CHEN
;
Lin-Hu GE
;
Xin XU
;
Nan-Shan ZHONG
;
- Publication Type:Journal Article
- Keywords:
Carcinoma,non-small-cell lung;
Molecular biology;
Immunohistochemistry;
Polymerase chain reaction
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze negative lymph nodes of 34 non-small cell lung cancer(NCLC) patients with total correction by means of fluorescent quantitation PCR and immunohistcchemistry,and to form molecular bi- ology staging.Methods Clinical data and tissue samples of 193 lymph nodes were collected from 34 patients under- going resection for non-small cell lung cancer.Using fluorescent quantitation reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemistry method,lymph nodes were examined for CEA gene mRNA,P53 and CK to form molecular biology staging.All the patients were followed-up for an average of forty months.Results The CEAmRNA was identified in 21.7% (42/193) lymph nodes negative patients from 17 patients(17/34,50%); TMN staging was up-regulated in 8 patients;positive lymph nodes were increased in 9 patients.P53 and AE1/AE3 were identified 9.8%(19/193) from 11 patients,18.6 % (36/193)from 15 patients,separately;TMN staging was up-regulated in 2 patients of P53 examination and 5 patients of AE1/AE3 analysis;positive lymph nodes were in- creased in in 7 patients of P53 examination and 11 patients of AE1/AE3 analysis.There was obvious statistical sig- nificance in them,but the molecular biology staging based on the three markers was not an independent factor on re- currence and metasis of lung cancer.Conclusion CEAmRNA.P53 and AE1/AE3 analysis could find lung cancer micrometasis more sensitively to form molecular biology staging which was relative to the prognosis,but not an inde- pendent prognostic indicator.It might be good to the therapy strategy after operation.
