RP-HPLC determination of valproate concentration in serum
- VernacularTitle:反相高效液相色谱法测定丙戊酸的血药浓度
- Author:
Yu-Xian LIN
;
Huang-Cai SU
;
- Publication Type:Journal Article
- Keywords:
Chromatography,high pressure liquid;
Valproic acid;
Serum concentration
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an RP-HPLC method for determining serum level of valproate.Methods A symmetryshield RP18 column (5?m,3.9mm?150mm) was used.The mobile phase,methanol-water(80:20) was used at a flow rate of 1.0ml/min.The detector was Waters 2487 dual?absorbance detector,which was set at the wavelength of 248nm.Serum sample was extracted with 2ml acetic n-hexane,then valproic acid in organic layer was derived by adding into?-bromoacetophenone,and cyclohcxanecarboxyiic acid was selected as an internal stan- dard.Results The calibration curve was linear in the range of 5.0 to 250.0vtg/ml.The average recovery rate was 98.23%,and the RSD within-day and between-day were both less than 3 %.Conclusion This method is suitable for therapeutic drug monitoring of phenytoin.
