Isolation,culture and identification of adipose derived stem cells from human subcutaneous adipose tissues
- VernacularTitle:人皮下脂肪来源脂肪干细胞的分离培养及鉴定
- Author:
Xun-Yi ZHANG
;
Min-Juan WU
;
Hou-Qi LIU
;
- Publication Type:Journal Article
- Keywords:
adipose tissue;
mesenchymal stem cells;
cell separation;
tissue engineering
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for isolating adipose derived stem cells(ADSCs)from resected human subcutaneous adipose tissues.Methods:ADSCs were isolated,cultured,and expanded from human subcutaneous adipose tissues.Immuno-fluorescent staining of specific molecules.FACS and multi-lineage differentiation induction were used to characterize the obtained ADSCs.Results:ADSCs obtained in this study had the characteristics of stem cells and expressed specific molecules;they also possessed a multi-lineage differentiation potential,which was genetically stable.Conclusion:ADSCs can be isolated from human subcutaneous adipose tissues,which provides a novel and abundant seeding cells for tissue engineering.
