A combined-staining method for neural cholinesterase and elastic and collagen fibers in rat pancreatic tissues
- VernacularTitle:大鼠胰腺组织神经胆碱酯酶、弹力纤维和胶原纤维的组合染色
- Author:
Shun-Min ZHANG
;
Jian-Ming ZHENG
;
Ming-Hua ZHU
;
Wei-Hong CHEN
;
- Publication Type:Journal Article
- Keywords:
nerve tissue;
cholinesterases;
elastin;
collagen;
staining and labeling
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the distribution of neural cholinesterase and elastic and collagen fibers in rat pancreatic tissues.Methods:Rats were decapitated and the pancreatic tissues were harvested,frozen and made into 8?m serial sections.A combined-staining method(K-R-VB-P-PA method,a combination of Karnovsky-Roots and Victoria blue-Ponceau-Picric acid) was used for staining and the distribution of neural cholinesterase and elastic and collagen fibers was observed.Results:The results showed that the neural cholinesterase was brown.the elastic fibers were cyan.the collagen fibers were light red.and the smooth muscle was yellow in the pancreatic acinar,islet of Langerhans and blood vessels.Conclusion:K-R-VB-P-PA combined staining method can demonstrate the distribution of neural cholinesterase,elastic and collagen fibers and smooth muscle fibers in the pancreatic tissues synchronously.
