Effects of antisense oligonucleotide of connective tissue growth factor on apoptosis of hypertrophic scar fibroblasts and the related mechanism
- VernacularTitle:结缔组织生长因子反义寡核苷酸体外对人增生性瘢痕成纤维细胞凋亡的影响
- Author:
Ming XU
;
Xin XING
;
- Publication Type:Journal Article
- Keywords:
cicatrix;
hypertrophic;
fibroblasts;
connective tissue growth factor;
oligonuc|eotides;
antisense;
apoptosis;
Fas
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of antisense oligonucleotide(ASODN)of connective tissue growth factor (CTGF)on the apoptosis of human hypertrophic scar fibroblasts(HSF)and the related mechanisms.Methods:The hypertrophic scar fibroblasts were cultured in vitro and were divided into the following 3 groups:CTGF-ASODN-treated group, liposome control group and blank control group.The distribution of CTGF-ASODN in HSF of different phases was observed under fluorescent microscope in 3 groups.Flow cytometer was employed to detect the apoptotic rate of HSF and the expression of Fas.The expression of CTGF,Fas and bcl-2 mRNA in HSF was assessed by RT-PCR.Results:24 h after transfection,large quantity of yellow-green fluorescence was seen in the HSF cytoplasm of CTGF-ASDON group in a scattered or doted manner, but not found in that of the other 2 groups.The apoptosis rate of HSF in CTGF-ASDON group was significantly higher than those in the other 2 groups(P
