HPLC determination of ursolic acid in Kangzhitong Jiangzhi Capsule
- VernacularTitle:高效液相色谱法测定康脂通降脂胶囊中熊果酸的含量
- Author:
Mei SHA
;
Wei XU
;
Li CHEN
;
- Publication Type:Journal Article
- Keywords:
Kangzhitong Jiangzhi Capsules;
Ursolic acid;
Determination;
HPLC
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for HPLC determination of ursolic acid in Kangzhitong Jiangzhi Capsule.Methods: HPLC,chromatographic column Kromasil C18(4.6mm?250 mm,5?m)and the mobile phase consisted of methyl alcoholwater-ammonium acetate-acetonitrile(9∶25∶0.8∶65) were used to find ursolic acid.The flow rate was 1.0ml?min and the detector wave-length was set at 215nm.Results: The calibration curve was in good linearity over the range of 64-320?g/ml(r=0.9996).The average recovery rate was 100.10%,with RSD=1.32%.Conclusion: This method is simple,accurate and reproducible so it can be used to determine the content of ursolic acid in Kangzhitong Jiangzhi Capsule.
