Studies on the effects of BSP over-expression in facilitating the metastasis of breast cancer cells to bone
- VernacularTitle:骨唾液蛋白(BSP)高稳定表达对乳腺癌骨转移的促进作用
- Author:
Hongyan DU
;
Jie WANG
;
Jing YANG
;
- Publication Type:Journal Article
- Keywords:
sialoglycoproteins;
breast neoplasms;
neoplasm metastasis
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The study was aiming at elucidating the rale of BSP over-expression in enhancing osseous metastasis of breast cancer cells,in order to search for new drug targets for prevention and treatment of such metastasis.Methods Chicken embryo allantois membrane(CAM)was used in the experiment.Breast cancer cells were inoculated on the superior surface of CAM sac,then the vascular tissues were collected in lower sac after incubation.The human specific Alu gene was amplified by PCR with genomic DNA extracted as template.Breast cancer cells were incubated with bone,which had becn treated with collagenase and trypsinase,and then OD_(488) of breast cancer was assessed to evaluate the adhesive affinity of breast cancer cells to bone.Results The findings of CAM assay and PCR amplifica- tion using human Alu primers and genomic DNA showed that Alu gene was amplified from 4 of 5 in MDA-MB-231BO(pI)group and 3 of 5 in MDA-MB-231BO(pID)group,but not amplified in control group.In bone adhesion assay,the OD_(488) of digested bone after MDA- MB-231BO(pI)and MDA-MB-231BO(pID)adhering to bone were 0.58?0.046 and 0.49?0.039,respectively,which were larger than that of MDA-MB-231BO(0.26?0.021,P
