Effects of ATRA on interacellular communication function and connexin 43 expressions in acute leukemia bone marrow stremal cells
- VernacularTitle:全反式维甲酸对急性白血病骨髓基质细胞GJIC功能的影响
- Author:
Yao LIU
;
Xi ZHANG
;
Zhongjun LI
;
- Publication Type:Journal Article
- Keywords:
acute leukemia;
bone marrow stromal cell;
connexin 43;
gap junction intercellular communication;
tretinoin
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of all-tram retinoic acid(ATRA)on intercellular communication function and connexin 43 expression in acute leukemia bone marrow stromal cells(ALBMSCs).Methods ALBMSCs were cultured in vitro,and the ATRA(1?10~(-5)mol/L)was added after serial subcultivation.The expression level of Cx43 was detected by immunochemistry and flow cytometry, and the gap junction intercellular communication(GJIC)was examined by using cell scarification with dye transfer(CSDT)technique.Re- sults Before and after addition of ATRA,the positive rates of Cx43 expression as detected by immunochemistry in ALBMSCs were(47.2?2.04)% and(54.5?5.86)%,respectively.The positive rates of Cx43 expression in ALBMSCs as detected by flow cytometry before and after the addition of ATRA were(38.75?23.95)% and(49.5?5.46)%,respectively.The expression of Cx43 in ALBMSCs after adding ATRA was significantly higher than that before the addition of ATRA(P
