Effect of blocking Wnt-?-catenin signaling pathway on activation of hepatic stellate cells
- VernacularTitle:阻断Wnt-?-catenin信号通路对肝星状细胞活化的影响
- Author:
Zhi-Hong WENG
;
Yan-Chang LEI
;
Cheng PENG
;
Shuling ZHANG
;
- Publication Type:Journal Article
- Keywords:
Liver;
Astrocytes;
Liver cirrhosis;
?-catenin;
Signal transduction
- From:
Chinese Journal of Infectious Diseases
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether functional Wnt-?-catenin signaling is present in activated hepatic stellate cells (HSC),and the effect of blocking this signaling on activation of HSC. Methods?-catenin expression in HSC was examined by immunocytochemistry.Wnt signalings in HSC-T6 were assessed using a T cell factor (TCF)-dependent luciferase reporter gene (pTOP- FLASH) assay.Wnt signalings in HSC-T6 were blocked by transfecting with a dominant negative TCF (dnTCF) expression plasmid,then the expression of alpha smooth muscle actin (?-SMA) and collagen typeⅠwere examined by Western blot.Results?-catenin staining was positive in the nuclei of HSC-T6.Luciferase activity in the cells transfected with pTOPFLASH was significantly higher than that in the cells transfected with pFOPFLASH (P
