The relationship between hepatitis B virus pre-S gene mutations and progression of liver disease
- VernacularTitle:乙型肝炎病毒前S基因变异与肝病进展的关系
- Author:
Jing-Juan DING
;
Mei WANG
;
Yue-Hui LIU
;
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Protein precursors;
Variation(genetics);
Polymorphism,restriction fragment length;
Sequence analysis;
Liver diseases
- From:
Chinese Journal of Infectious Diseases
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between hepatitis B virus(HBV)pre-S gene mutations and progression of liver disease.Methods The entire pre-S1,pre-S2 genes were amplified hy nested polymerase chain reaction(PCR)and the products were digested by NlaⅢ.The method for detecting pre-S2 start codon mutation was established based on the digested restriction fragment length polymorphism(RFLP).Pre-S gene deletion was revealed by electrophoresis on polyacrylamide gel(PAGE).Pre-C A1896 and basic core promoter T1762/A1764 mutations were identified by direct sequencing of PCR products.The 138 sera from patients with HBV-related disease,including asymp- tomatic carriers(ASC),chronic hepatitis(CH),liver cirrhosis(LC),hepatocarcinoma(HCC),were tested by these methods.Results The detection rate of pre-S deletion mutation was higher in patients with HCC(56.3%)and LC(42.9%)than those with CH(11.8%)and ASC(8.1%,P
