Genetic typing and antibiotic susceptibility testing of strains from pseudomonas aeruginosa keratitis patients
- VernacularTitle:铜绿假单胞菌性角膜炎的细菌基因分型与体外药物敏感性研究
- Author:
Dan WANG
;
Qing-Feng LIANG
;
Zhi-Qun WANG
;
Ran LI
;
Xu-Guang SUN
;
- Publication Type:Journal Article
- Keywords:
keratitis;
pseudomonas aeruginosa;
antimicrobial susceptibility;
genotype
- From:
Ophthalmology in China
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the characteristics of Genetic typing and the antibiotic susceptibility testing of strains from Pseudomonas aeruginosa keratitis patients.Design Experimental study,Participants 23 eyes of 23 patients of Pseudomonas aeruginosa keratitis.Methods The genomic was extracted and amplified with PCR.The PCR products were purified and sequenced.The results were registered in MIST web Antibiotic susceptibility testing were performed in theses strains,Main Outcome Measures Sequence types and antibiotic susceptibility.Results The isolates were resolved into 20 STs.Two lineages were identified.MIC test showed that strains were more susceptible to aminoglycosides,The activity of quinolones and cephalosporin were higher than that of aminoglycosides.Conclusion MIST can determine homology of the strains from Pseudomonas aeruginosa keratitis by clustering results. There is no finding about relationship between Genetic typing and drug resistance.
