Expression of 15-Lipoxygenase isoenzymes in the pulmonary arteries during hypoxia
- VernacularTitle:缺氧对15-脂氧酶同工酶在肺动脉中表达的影响
- Author:
Ye LIU
;
Wei-Na HAN
;
Shou-Li GUO
;
Chang-Lian LV
;
DA-LING
;
- Publication Type:Journal Article
- Keywords:
15-lipoxygenase;
isoenzyme;
pulmonary arteries;
hypoxia;
protein;
mRNA
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim The purpose of this study was to compare the differential expression of 15-lipoxygenase isoenzymes in the pulmonary arteries between normoxia and hypoxia and to explore their roles in the formation of hypoxic pulmomary vasoconstriction. Method Eighteen SD rats were randomly divided into two groups(n=9):the normoxic control group breathing fresh gas and the hypoxic group breeding in animal hypoxic incubator.Immunohistochemical method,in situ hybridization and Western blot were employed to determine certain 15-lipoxygenase isoenzymes which involved in the process of hypoxic pulmonary vasoconstriction.Results ①In normoxic control group,the expression of 15-LO-1 protein was detected in the pulmonary arteries;but the expression of 15-LO-2 protein wasn’t detected.②The expression of 15-LO-1 protein in hypoxic group was much stronger than that in normoxic group (P
