Expansion of CD4~+ CD25~+ FoxP3~+ regulatory T cell pool in patients with active tuberculosis
- VernacularTitle:CD4~+CD25~+FoxP3~+调节性T细胞与结核病的关系
- Author:
Bo-Ping ZHOU
;
Xin-Chun CHEN
;
Mei-Zhong LI
;
Qun-Yi DENG
;
Xiao-Hua LE
;
Chi WU
;
Wei-Ye YU
;
Wei ZHANG
;
Huo-Sheng WANG
;
Xiang-Dong FU
- Publication Type:Journal Article
- Keywords:
CD4CD25-positive T-lymphocytes;
Tuberculosis,pulmonary;
Tuberculosis,lymph node;
Real-time reverse transcriptase polymerase chain reaction;
Flow cytometry
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the frequency of CD4~+CD25~+FoxP3~+regulatory T cells (Treg)and the expression of the functional protein,FoxP3,in patients with active tuberculosis and the relationship between Treg and the pathogenesis of tuberculosis.Methods Forty-five patients with active tuberculosis(including 25 cases of pulmonary tuberculosis and 20 tuberculous lymphadenitis), 20 healthy controls,20 recovered tuberculosis patients and 6 patients with reactive hyperplasia in cer- vical lymph node were enrolled.The frequency of CD4~+ CD25~+ FoxP3~+ Treg in the peripheral blood was measured by flow cytometry.FoxP3 mRNA expression was determined by real-time reverse transcriptase-polymerase chain reaction(RT-PCR)and the expression of FoxP3 protein in lymphoid tissues was measured by immunohistochemistry.Results The frequency of natural Treg in the peripheral blood from the patients with active tuberculosis was 2.91%?0.23%,which was signifi- cantly higher than that of healthy control group(1.22%?0.18%)and recovered tuberculosis patients(1.50%?0.17%,P
