A study on inflammatory reaction induced by N-protein of severe acute respiratory syndrome-CoV in human alveolar type Ⅱ epithelial cell(A549)
- VernacularTitle:严重急性呼吸综合征病毒N蛋白致人Ⅱ型肺泡上皮细胞(A549)的炎症反应
- Author:
Dong HAO
;
Li-Xian HE
;
Jie-Ming QU
;
Yu PAN
;
Bi-Jie HU
;
- Publication Type:Journal Article
- Keywords:
Severe acute respiratory syndrome;
SARS virus;
Viral proteins;
Pulmonary alveol;
Epithelial cells;
Inflammation
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study inflammatory reaction induced by N-protein of severe acute respiratory syndrome(SARS)-coronavirus(CoV)in human alveolar typeⅡepithelial cell(A549). Methods Effects on growth of A549 cell by N-protein of SARS-CoV:activity of A549 cells was determined by thiazylyl blue colorimetry assay at 24,48,72 and 96 h,respectively.Effects on cyto- kine production by A549 cells exposed to N-protein of SARS-CoV:interleukin(IL)-6,IL-10 and transforming growth factor-?1(TGF-?1)concentration in culture supernatant were determined by enzyme-linked immunosorbent assay(ELISA).Effects on mRNA expression of cytokine of A549 cells and matrix metalloproteinases-9(MMP-9)exposed to N-protein of SARS-CoV:total RNA of A549 cells was extracted using Rneasy mini kit;RT-PCR was employed to measure the mRNA expression of IL-6,IL-10,TGF-?1 and MMP-9 semiquantitatively.Results Different concentrations of N-protein could all inhibit the growth of A549 cells(after 48 h)and the inhibition by 20?g/mL pro- tein was the strongest.Compared with the control group(0.737?0.024,0.968?0.007),the A val- ues of experimental groups at 72 h and 96 h(0.672?0.027,0.799?0.092)decreased obviously (P
