Study on the expression of Toll-like receptor 4 in peripheral blood mononuclear cell of patients with chronic hepatitis B virus infection
- VernacularTitle:Toll样受体4在慢性乙型肝炎病毒感染者外周血单个核细胞中的表达及意义
- Author:
Hui WANG
;
Wen-Jun DU
;
Lai-Ying QIN
;
Liang-Wen LIU
;
Shi-Jun CHEN
;
- Publication Type:Journal Article
- Keywords:
Hepatitis B,chronic;
Receptors,cell surface;
Flow cytometry;
Reverse transcriptase polymerase chain reaction
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of Toll-like receptor(TLR)4 protein and the transcription of TLR4 mRNA in the peripheral blood mononuclear cells(PBMC)from patients with hepatitis B virus(HBV)infection and explore the relationship between TLR4 and chronic HBV infec- tion.Methods The expression level and transcription level of TLR4 were determined by flow cytometre and reverse transcriptase polymerase chain reaction respectively in PBMC from 37 chronic hepatitis patients,28 liver cirrhosis patients,31 severe hepatitis patients and 27 healthy controls. Meanwhile,liver function,as well as blood routine test,prothrombin test activity(PTA)and HBV DNA was measured.Results The expression level and transcription level of TLR4 in patients were higher than those in healthy controls(P
