Study on HPLC Fingerprint of Smilacis Rhizoma
- VernacularTitle:土茯苓药材HPLC指纹图谱研究
- Author:
Lei LI
;
Honggui ZHANG
;
Yikun SUN
;
Yanjiang QIAO
;
- Publication Type:Journal Article
- Keywords:
smilacis rhizoma;
high efficiency liquid chromatography(HPLC);
fingerprint
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Adopting RP-HPLC method,to study and establish the fingerprint of smilacis rhizome.Methods: YMC-Pack ODS-A(5 ?m,4.6 mm?250 mm) chromatographic column,acetonitrile-0.05% phosphoric acid solution gradient elution were applied;with flow rate of 0.8 ml/min and UV detector at 290 nm.Results: Technology investigation indicated that the analytical method this study established has a better reproducibility.The similarity of smilacis rhizoma fingerprints from different sources is better;smilacis rhizome and its three confusions fingerprints are apparently different.Conclusion: HPLC fingerprint analysis can be a method for quality control of medical material of smilacis rhizoma.