Experimental study on effect of arsenic trioxide on vascular smooth muscle cells
- VernacularTitle:三氧化二砷纳米微粒对抑制兔血管平滑肌细胞增殖作用的实验研究
- Author:
Qin LU
;
Yan-Li AN
;
Zi-Yu WANG
;
Dong-Sheng ZHANG
;
Huan-Zhang NIU
;
Juan-Juan FANG
;
Gao-Jun TENG
;
- Publication Type:Journal Article
- Keywords:
Arsenic trioxide;
Muscle,smooth,vascular cells;
Radiology,interventional
- From:
Chinese Journal of Radiology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of arsenic trioxide(As_2 O_3)nanoparticles on rabbit vascular smooth muscle cells in vitro in comparison with normal form As_2 03.Methods The rabbit vascular smooth muscle cells were cultured in vitro.Nano and normal forms of As_2O_3 with drug concentrations of 3?mol/L were added into the cells.Cell proliferation curve was drawn according to the light absorption values of MTT test.Flow cytometry was applied to observe the apoptosis.DNA was extracted and underwent electrophoresis.Results Cell proliferation treated with the 3?mol/L concentration of As_2O_3 was inhibited. Cell growth was inhibited markedly with increased treatment time,and the inhibition effect of nano drug form seemed stronger than that of normal form.MTT light absorption values of cells treated at 24,48 and 72 h showed statistically significant difference(H=10.934,15.039,15.539,P
