Biological effect of hepatocyte growth factor gene transfection to rat cerebral ischemic model:the experimental study
- VernacularTitle:肝细胞生长因子基因转染大鼠急性脑卒中模型的生物效应
- Author:
Xiao-Bo ZHANG
;
Zheng-Yu JIN
;
Ming-Li LI
;
Ren-Zhi WANG
;
Gui-Lin LI
;
Yan-Guo KONG
;
Jian-Ming WANG
;
Shan GAO
;
Hong-Zhi GUAN
;
De-Tian WANG
;
Yufeng LUO
;
- Publication Type:Journal Article
- Keywords:
Hepatocyte growth factor;
Brain ischemia;
Mice,transgenic
- From:
Chinese Journal of Radiology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate hepatocyte growth factor(HGF)gene expression and biological effect after gene transfection into penumbra tissue in rat cerebral ischemic model.Methods Human HGF cDNA was ligated to pIRES2-EGFP vector.The recombinant plasmid was transfected into the penumbra tissue with liposome.Brains of treated and control animals were analyzed 7 days later.Expression of HGF protein was determined by fluorescence microscopy and immunohistochemistry.Vessel numbers were quantified.Changes of cerebral blood flow(CBF)was detected by CT perfusion.Results Enzymatic digestion and electrophoresis confirmed that HGF fragment had been correctly cloned into BamH I and Sal I sites of pIRES2-EGFP.After HGF gene transfection,expression of HGF in transfected neurocytes was observed with fluorescence microscopy and immunohistochemistry.The number of vessels was significantly increased in penumbra tissue transfected with HGF vector as compared with control vector(46.71?7.11, 20.43?3.21,18.00?3.27,respective,F = 74.447;P
