INFLUENCE OF HEAT STRESS ON AMPK ACTIVITY AND LIPID METABOLITES OF
- VernacularTitle:热应激对体外仔猪肝细胞AMPK活性及脂质代谢产物的影响
- Author:
Ping ZHENG
;
Dai-Wen CHEN
;
Ke-Ying ZHANG
;
Bing YU
;
- Publication Type:Journal Article
- Keywords:
AMP-activated protein kinase;
piglet hepatocyte culture;
lipid metabolism;
heat stress
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influence of high cell incubating temperature on AMP-activated protein kinase(AMPK) activity and lipid metabolites of piglets hepatocytes in vitro.Method: Primary hepatocytes of piglets about age 55d were separated and cultured under 37 ℃(control) or 42 ℃(heat stress).The anabolic and catabolic products of [14C]-oleic acid were detected for hepatocytes and culture media at 60min,120min and 180min.There were 9 replicates per time point.Result: Heat stress activated AMPK activity and enhanced fatty acid oxidation.The production of [14C]-CO2 and [14C]-acid soluble metabolites(ASM) was higher in heat stress group than in the control.At the same time,heat stress depressed the incorporation of [14C]-oleate into phospholipids,monoglycerides,triglycerides,cholesterol and cholesteryl ester.Conclusion: Heat stress activated AMPK activity and enhanced the formation of anabolic products and depressed catabolic products in piglets hepatocytes in vitro.
