Construction of serine protease gene mutant and study on pathogenicity of serine protease gene of Entero-coccus faecalis
- VernacularTitle:粪肠球菌丝氨酸蛋白酶基因突变株的构建和致病性研究
- Author:
Li-Xian WU
;
Wen HUANG
;
Sun XIANG
;
Ping XIAO
;
- Publication Type:Journal Article
- Keywords:
Enterococcus faecalis;
Serine endopeptidases;
Mutantion;
Peritonitis;
Endocar-ditis;
Disease models,animal
- From:
Chinese Journal of Infectious Diseases
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the serine protease gene(sprE)mutant and to study the pathogenicity of sprE gene of Enterococcus faecalis.Methods Recombinant suicide vector pCQ001 of Enterococcus faecalis with pTX4577,was constructed.Then,created isogenic sprE-deficient mu- tant(*sprE)by allelic replacement was constructed.Moreover,the growth ability and the virulence of the mutant were compared with those of the wide type in vitro and in vivo respectively.A mouse peritonitis model and a rabbit endocarditis model were utilized in the study.Results The *sprE was selected by kanamycin and identified by polymerase chain reaction(PCR),pulsed field gel electropho- resis(PFGE)and Southern blot.The evidences showed that the sprE gene had a major role in helping bacteria to resist the elevated temperature and oxidative stress.The virulence of mutant decreased af- ter sprE gene was knocked out.Conclusions The *sprE of Enterococcus faecalis is constructed suc- cessfully,sprE gene is important in the pathogenesis of Enterococcus faecalis,which probably is a major virulence factor of Enterococcus faecali.
