Expression and Purification of the Fusion Protein Anti-epilepsy Peptide/Glutathiones-transferase
- VernacularTitle:抗癫痫肽/GST融合表达及包涵体蛋白复性纯化
- Author:
De'An ZHANG
;
Zongren WANG
;
Zhongjun SHAO
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
anti-epilepsy peptide;
fusion protein;
inclusion body;
purify
- From:
Journal of Zhejiang Chinese Medical University
2007;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
[Aim] To gain the fusion protein purified GST-AEP.[Methods] The protein GST-AEP was expressed in E.Coli-DH5? as a fusion protein induced by IPTG.The protein was a kind of inclusion body.The purifying and refolding to inclusion body were optimized.The purity of GST-AEP was identified by 12% SDS-PAGE and thin-layer scanning analysis.The quantitation of the fusion protein GST-AEP was done with BCA Protein Assay.[Results] Purity of GST-AEP was higher than 90% and concentration was about 0.163?g/?l.[Conclusion] The fusion protein was highly purified and the method of fusion protein purification from the inclusion body was developed,which was the basis for further study on AEP.
