Role of bone marrow mesenchymal stem cells in the recovery of rat acute renal tubular damage
- VernacularTitle:骨髓间质干细胞对大鼠急性肾小管损伤修复的促进作用
- Author:
Xiang LIU
;
Xue-Qing YU
;
Jing NIE
;
Zhi-Jian LI
;
Xin AN
;
Xiao-Yan LI
;
Wen-Xing PENG
;
Xiu-Qing DONG
;
- Publication Type:Journal Article
- Keywords:
Kidney failure,acute;
Mesenchymal stem cells;
Kidney tubules;
Grow factors;
Inflammatory factors
- From:
Chinese Journal of Nephrology
2005;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether mesenchymal stem cells can promote the recovery of acute renal tubular damage induced by mercuric chloride and to explore its possible mechanism.Methods Acute renal failure rat model was established by intraperitoneal injection of mercuric chloride.SD rats were randomly divided into three groups which were MSCs injection group, saline infusion group and normal control group.Seven days later,the changes of rat weight,survival,renal function and pathology were observed;PCNA,ED-1 and GFP were detected by immunohistochemistry; The expression of cytokines in kidney and the distribution of GFP plasmid-transfected MSCs in kidney were examined by RT-PCR.Results MSCs infusion ameliorated the decline of rat weight,survival, renal function,and pathological changes.PCNA and ED-1 positive cells in MSCs group were fewer than those in saline group.Expression of growth factors EGF,PDGF,HGF were obviously up- regulated and pre-inflammatory cytokines TNF-?was significantly reduced in MSCs-treated kidneys. GFP-labelled MSCs occurred occasionally in renal interstitium of MSCs-treated rats,but not in renal tubules.Conclusions Bone marrow mesenchymal stem cells can promote the recovery of acute renal tubular epithelial cells damage caused by mercuric chloride.The mechanism may partly depend on regulating the excretion of cytokines in renal microenvironment rather than completely depend on their differentiation to tubular cells.
