Quantitative analysis of the patterns of heat shock protein 70 expression induced by Hantaan virus infection in vitro
- VernacularTitle:汉滩病毒体外诱导热休克蛋白70的表达
- Author:
Lu YU
;
Heng MA
;
Chun-Guang DUAN
;
- Publication Type:Journal Article
- Keywords:
Hantaan virus;
Heat-shock proteins 70;
Heat-shock response
- From:
Chinese Journal of Infectious Diseases
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the pattern and quantify the heat shock protein HSP)70 and HSP70 mRNA in Vero-E6 cells after infection with Hantann virus(HTNV).Methods The expres- sion of HSP70 and change of its mRNA level were detected by immunocytochemical staining,nucleic acid hybridization in situ and RT-PCR.Results In situ hybridization and RT-PCR were used to eval- uate the level of HSP70 mRNA during Hantaan 76-118 infection.HSP70 mRNA increased 0.5 h after infection,reached its peak by 12 h and gradually declined to steady state level by 72 h(vs.sham infec- ted group,P＜0.05).The expression of HSP70 protein induced by Hantaan 76-118 infection was e- valuated by quantitative immunocytochemical staining.HSP70 increased 0.5 h after infection,reached its peak by 12 h and decreased at 72 h after infection(vs.sham infected group,P＜0.05).Conclu- sions HSP70 can be induced directly by HTNV infection at both mRNA and protein levels,It pro- vides a basis for the further study of the pathogenesis,prevention and treatment of hemorrhagic fever with renal syndrome(HFRS).
