The Change and the Mechanism of bcl-6 Expression in K562 Cells Inducing Differentiation Along Different Lineage Cells.
- VernacularTitle:K562细胞向不同细胞系分化时bcl-6表达变化及其机制
- Author:
Yongqing ZHANG
;
Gaosheng HUANG
;
Xiequn CHEN
;
- Publication Type:Journal Article
- Keywords:
K562 cells;
Differentiation;
bcl-6 gene;
Mutation
- From:
Journal of Medical Research
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes and the mechanism of bcl-6 expression in K562 cells inducing differentiation along different Myelocyte-lineage cells,and to investigate the relationship between expression of bcl-6 and directional differentiation of K562 cells.Methods Models of K562 cells inducing differentiation along megakaryocyte-lineage,erythrocyte-lineage and macrophagocyte- lineage were established with inducers TPA(tetradecanoylphorbol 13-acetate),Hu(hydrozyurea)and HMBA(hexamethylene bisacet- amide)respectively,Western blot assay was applied to detect the expression of bcl-6 in K562 cells before and after the induction.Mean- while,PCR,cloning and direct DNA sequencing were used to identify mutations in the 5'regulatory region of bcl-6 in K.562 cells induced with TPA.Results Up-regulation of bcl-6 expression was found only in K562 cells inducing differentiation along megakaryocyte-line- age,and mutation of noncoding regulatory region of bcl-6 gene was not found.Conclusions bcl - 6 may be the key gene to regulate K.562 cells directional differentiation along different myelocyte-lineage ceils,the up-regulation of bcl-6 expression could play an important role in K562 cells inducing differentiation to megakaryocydte-lineage,and the change of bcl-6 expression might be not related with the mutation of the gene's 5'regulatory region.
