Study on the ?-lactamases in eight pan-drug-resistant citrobacter freundi isolates
- VernacularTitle:泛耐药弗劳地柠檬酸杆菌产?内酰胺酶及同源性
- Author:
Jun LUO
;
De-Mei ZHU
;
Xiao-Gang XU
;
- Publication Type:Journal Article
- Keywords:
Citrobacter freundii;
Drug resistance;
bacterial;
Metallo proteases;
beta-lactamases
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Study the production of the?-lactamases in eight pan-resistant citrobact- er freandi and their epidemic characteristics.Methods Modified Hodge test and EDTA-disk syner- gy test were used to screen Metallo-?-lactarnases,and isoelectric focusing to estimate the production of ESBLs and AmpC enzymes.ESBLs,AmpC,MBL genes were amplified and analyzed by PCR and DNA sequencing.Molecular epidemics were investigated by PFGE.Results All the 8 strains pro- duced TEM-1,CTX-M-14,4 of them at the same time producing CTX-M-3.No Plasmid mediated AmpC was found.Furthermore,a new subtype of MBL with one amino acid different from IMP-8 was de- tected.All the 8 strains belong to the same PFGE type.Conclusions Pan-drug-resistant citrobacter freundi produced more than one kind of?-lactamases.All these isolates were clonally related.
