Clinical and Experimental Study on Human Bocavirus in Children with Lower Respiratory Tract Infections.
- VernacularTitle:小儿人博卡病毒感染临床与实验研究
- Author:
Feng LIN
;
Aiping ZENG
;
Ningmin YANG
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Human Bocavirus;
Infection;
Real time PCR;
Western blot
- From:
Journal of Medical Research
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To characterize the assay method and the clinical symptoms of the infant infection of the human bocavirus in order to advance the diagnositic and therapeutic technology for human bocavirus. Methods The DNA and specific antibody of human bocavirus from pharyngeal extracts, nasopharyngeal swab and serum were detected using real time PCR and Western blot. The overall diagnosis and analysis of the clinical symptoms, sign and conventional experimental test were performed, and meanwhile the long-term follow-up was done. Results 7 specimens were detected as human bocavirus positive from 240 pharyngeal extracts (7/240), and the positive ratio was 2.92%. The clinical symptoms were mainly high fever and temporary cough. and the experimental index of peripheral blood leucocyte, C-reaction protein, blood sedimentation and hemobiochemistry were usually in normal region. No specific characteristic was observed by the thoracic X-ray. and cardio-pulmonary function was normal through the long-term investigation. Conclusions Human bocavirus was one of pathogens inducing children lower respiratory tract infections. The real time PCR and Western blot were sensitive and specific detection technologies.
