Osteogenic action of human osteoblasts and alloplastic decalcified cancellous bone
- VernacularTitle:人成骨细胞与异体脱钙松质骨成骨作用的实验研究
- Author:
Guang-He WEN
;
Xiao-Bin HUANG
;
Gang CHEN
;
Shu-Chun LI
;
Jun ZHO
;
- Publication Type:Journal Article
- Keywords:
Osteoblast;
Alloplastic decalcified cancellous bone
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study osteogenic courses and features of human osteoblasts cultured in vitro and seeded into alloplastic decalcified cancellous bone.Methods Human osteoblasts isolated from normal human perios- teum were cultured and amplified in vitro.Thy configuration and developing manners of osteoblasts were observed. Then osteoblasts were seeded into alloplastic decalcified cancellous bone.Thy composites were implanted and cultured in thy body of athymic mice.The specimens were obtained 8 weeks later.Osteogenic characteristics were observed by scanning electron microscope.Results Osteoblasts isolated from human periosteum developed well and proliferated rapidly in vitro.Island new bone formation could be observed histologically 8 weeks after the composites of os- teoblasts and alloplasric decalcified cancellous bone were implanted and cultured in the body of athymic mice.Conclu- sion Calcified bone can be formed by seeding osteoblasts into alloplastic decalcified cancellous bone,which may be a new kind of bone graft source to construct bone defects.
